Citation: S. Lueck (2022-02-22): Classified microscopy image data set of powdery mildew-infected barley leaves at 48hai, containing positive images with fungal microcolonies and negative examples. DOI:10.5447/ipk/2022/1

Abstract: This dataset contains Z-stacked cutouts of full-slide microscopy images of barley leaves 48 hours after infection with barley powdery mildew. The set includes 9877 positive samples containing fungal microcolonies and 8069 negative samples without colonies. Detached barley leaves were inoculated with Blumeria graminis f. sp. hordei. At 48 hours after inoculation (hai), the fungal growth was stopped by incubating the leave segments in a clearing solution (7 mL 96% Ethanol and 1 mL Acetic acid) for 48 hours at room temperature. After that, the fungal colonies were stained with Coomassie staining solution (0.3% Coomassie R250, 7.5% (w/v) trichloroacetic acid, and 50% (v/v) methanol) for 5 minutes and then washed several times with water. The prepared samples were mounted on microscope slides with 50 % glycerol to avoid drying the leaves during image acquisition. The microscopy image data were acquired on a Zeiss AxioScan.Z1 high-performance microscopy slide scanner and ZEN 3.0 (blue edition) software (Carl Zeiss AG). The imaging was done in a bright field configuration with a Hitachi HV-F202SCL camera (3 CCD 1/1.8" progressive scan color sensor with 1600x1200 effective pixels and 24-bit color depth), 1x camera adapter and scanned with EC Plan-Neofluar 5x/0.16 M27 with 0.16 NA objective. The acquired image data was stored in a CZI file container that combines all relevant image and meta information in one file. The raw images were analyzed with the BluVisionMicro analysis software (version 1.1; https://github.com/snowformatics/BluVisionMicro) and described in ISA-tab format.

License: CC BY 4.0 (Creative Commons Attribution)

DOI: 10.5447/ipk/2022/1

Content: 2 Directories 17949 Files (1.8 GB)