Citation: A. Câmara (2021-02-24): Simulation of chromosome condensation with loop extruders and centromeric nucleosomes. DOI:10.5447/ipk/2021/5

Abstract: This dataset contains four movies showing the condensation of a chromosome with centromeric nucleosomes via loop extrusion. HolocentricChromosome_noEffectCentromeres.mp4 - Condensation of a holocentric chromosome, where the centromeric nucleosomes have no effect on the Loop Extruders. Three visualization modes are present. Chromatin is shown as a 10 nm thick grey fibre, the Loop Extruders are represented by the bound nucleosomes as pairs of yellow beads and the centromeric nucleosomes are shown as beads coloured in red or in a gradient from blue to red, following their chromosome position, as indicated by the subtitles. The simulation presents 1000 LEs and 100 centromeric nucleosomes. HolocentricChromosome_blockingCentromeres.mp4 - Condensation of a holocentric chromosome, where the centromeric nucleosomes have a blocking effect on the Loop Extruders. Three visualization modes are present. Chromatin is shown as a 10 nm thick grey fibre, the Loop Extruders are represented by the bound nucleosomes as pairs of yellow beads and the centromeric nucleosomes are shown as beads coloured in red or in a gradient from blue to red, following their chromosome position, as indicated by the subtitles. The simulation presents 1000 LEs and 100 centromeric nucleosomes. HolocentricChromosome_anchoringCentromeres.mp4 - Condensation of a holocentric chromosome, where the centromeric nucleosomes have an anchoring effect on the Loop Extruders. Three visualization modes are present. Chromatin is shown as a 10 nm thick grey fibre, the Loop Extruders are represented by the bound nucleosomes as pairs of yellow beads and the centromeric nucleosomes are shown as beads coloured in red or in a gradient from blue to red, following their chromosome position, as indicated by the subtitles. The simulation presents 1000 LEs and 100 centromeric nucleosomes. MonocentricChromosome_anchoringCentromeres.mp4 - Condensation of a monocentric chromosome, where the centromeric nucleosomes have an anchoring effect on the Loop Extruders. Three visualization modes are present. Chromatin is shown as a 10 nm thick grey fibre, the Loop Extruders are represented by the bound nucleosomes as pairs of yellow beads and the centromeric nucleosomes are shown as beads coloured in red or in a gradient from blue to red, following their chromosome position, as indicated by the subtitles. The simulation presents 1000 LEs and 20 centromeric nucleosomes.

License: CC0 1.0 Universal (Creative Commons Public Domain Dedication)

DOI: 10.5447/ipk/2021/5

Content: 0 Directories 4 Files (252.3 MB)