Citation: J. Szymanski et al. (2020-07-28): Analysis of Wild Tomato Introgression Lines Elucidates the Genetic Basis of Metabolome Variation in a Ripening Tomato Fruit. DOI:10.5447/ipk/2020/22

Abstract: LC-MS analysis of the Introgression Lines (ILs; Eshed & Zamir 1995) and Backcrossed Inbred Lines (BILs; Ofner et al. 2016) developped from the wild Peruvian accession of Solanum pennellii in the genetic background of the Solanum lycopersicum cultivar M82. Fruit at the breaker and red ripe stages of ILs and BILs were harvested from the field at the Western Galilee Experimental Station in Akko, Israel. Samples represent methanol extracts analysed using a high-resolution UPLC/qTOF system comprised of a UPLC (Waters Acquity) which was connected online to a photodiode array (PDA) and qTOF detectors. Details on plant material and experimental protocols are provided in attached Methods file. Attached Metadata file provides all details on sample identity, measurement batches and quality controls. The analysis has been performed at the Department of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, Israel in the lab of Prof. Asaph Aharoni. Please visit our GitHub repository (https://github.com/NAMlab/kILBIL) for data processing, annotation and analysis scripts, as well as list of studies using the dataset, and links to further transcriptomic and phenomic data resources for the respective genotypes. Eshed, Y. & Zamir, D. An introgression line population of Lycopersicon pennellii in the cultivated tomato enables the identification and fine mapping of yield-associated QTL. Genetics 141, 1147–1162 (1995). Ofner, I., Lashbrooke, J., Pleban, T., Aharoni, A. & Zamir, D. Solanum pennellii backcross inbred lines (BILs) link small genomic bins with tomato traits. Plant J. 87, 151–160 (2016).

License: CC BY-SA 4.0 (Creative Commons Attribution-ShareAlike)

DOI: 10.5447/ipk/2020/22

Content: 3 Directories 4647 Files (864.5 GB)