Citation: S. Kale (2021-10-26): Annotated resistance gene enrichment sequencing (RenSeq) assemblies of a winter wheat diversity panel. DOI:10.5447/ipk/2022/4

Abstract: RenSeq assemblies of 907 diverse T. aestivum genotypes and two control genotypes. The assemblies were generated using CLC assembler (https://digitalinsights.qiagen.com/products-overview/discovery-insights-portfolio/analysis-and-visualization/qiagen-clc-assembly-cell/). The contigs from each accession were annotated with AUGUSTUS v3.3.1 using wheat gene models as training datasets and contigs with complete genes were identified. Amino acid (AA), coding sequence (CDS) and transcript sequence for each complete gene was extracted using getAnnoFasta.pl script from AUGUSTUS package. The files ending with “_updated_fasta” is the direct output of CLC assembler and contains contigs from each accession. The files ending with “_updated_complete.gff”, “_updated_complete.mrna”, “_updated_complete.aa” and “_updated_complete.codingseq” contain gene information in GFF3 format, transcript sequences, amino acid sequences and coding sequences respectively from each accession. Passport data and/or pedigree information and BioSamples IDs of RenSeq raw data are provided in the sample information file.

License: CC BY 4.0 (Creative Commons Attribution)

DOI: 10.5447/ipk/2022/4

Content: 5 Directories 4546 Files (503.2 GB)