Citation: M. Mascher (2015-01-23): Barley gene annotation file of near-complete high-confidence genes with open reading frame information. DOI:10.5447/ipk/2015/0

Abstract: To avoid annotation artifacts due to fragmented gene models, we generated a GFF files of barley genes (PMID: 23075845) whose protein sequences is nearly completely represented in the Morex WGS assembly. Protein sequences were aligned to the genomic contigs with exonerate (PMID: 15713233). Genes were considered near-complete if 98 % of their protein sequences could be aligned to the genomic sequence. A total of 18,039 (74 %) out of 24,243 high-confidence genes positioned on the Morex WGS assembly had near-complete ORFs.

License: CC BY 4.0 (Creative Commons Attribution)

DOI: 10.5447/ipk/2015/0