Citation: M. Mascher (2015-02-19): Genotyping-by-sequencing SNP matrix of 243 diverse barley accessions. DOI:10.5447/ipk/2015/1

Abstract: Genotyping-by-sequnencing (GBS) libraries were constructed for 243 barley accession. Raw reads were trimmed with with cutadapt and mapped to the draft genome assembly of barley cultivar ‘Morex’ with BWA-MEM using default parameters. SNPs were called with SAMtools 0.1.19 and filtered with an AWK script. The minimum read depth for a successful genotype calls was 10. SNPs with more than 5 % missing genotype calls were discarded. The resultant genotype matrix is accessible here as an MS Excel file.

License: CC BY 4.0 (Creative Commons Attribution)

DOI: 10.5447/ipk/2015/1