Citation: WHEALBI Consortium (2019-04-30): Single-nucleotide polymorphism matrices for 403 barley genotypes (WHEALBI diversity panel). DOI:10.5447/ipk/2019/5

Abstract: Single-nucleotide polymorphism (SNP) matrices for a diversity panel of barley (Hordeum vulgare L.) accessions belonging to the WHEALBI panel. Exome sequences that revealed substantial variation in the barley genepool were obtained and validated. Data were successfully derived for 403 genotypes from the WHEALBI research project (EU FP7 no. FP7-613556), comprising formally bred cultivars, landraces and wild barley lines. This source material represents a range of worldwide barley genetic diversity, assembled to quantify variation and explore adaptation, and possible breeding responses (http://www.whealbi.eu/). The reference genome sequence assembly of barley cv. Morex (Mascher et al. 2017, Nature [doi:10.1038/nature22043]) was used as a reference. More than 64 million SNPs were extracted from sequences, which after applying a quality criterion of at least 80 % of genotypes being represented at a SNP locus was reduced to just under 2.1 million SNPs. All seven of barley’s chromosomes (1H to 7H) were well covered, with a range of 262,014 (6H) to 334,501 (2H) polymorphisms and a median distance between SNPs of between 14 and 27 bp, although a few large gaps in coverage remained, especially close to centromeres. Further details can be read in Bustos-Korts et al. 2019, The Plant Journal.

License: CC BY 4.0 (Creative Commons Attribution)

DOI: 10.5447/ipk/2019/5