Abstract: The published scripts are integrated in a pipeline which was developed at the IPK in the frame of a master thesis. The pipeline aims to perform a gene prediction in genomic data using additional information from RNA-seq data. The process is divided into four main steps that integrate several external tools: 1) RNA-Seq-Scaffolding (L_RNA_Scaffolder), 2) repeat masking (Kmasker), 3) RNA-seq alignment (STAR or HISAT2) and 4) gene prediction (GeMoMa). The pipeline is developed to work at the computing infrastructure of the IPK.
License: CC BY 4.0 (Creative Commons Attribution)
DOI: 10.5447/ipk/2017/0
| CONTRIBUTOR: | |
| CREATOR: |
Chris Ulpinnis
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| PUBLISHER: | Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, Stadt Seeland, Corrensstraße 3, 06466, Germany |
| SIZE: | 0 B |
| SUBJECT: | RNA scaffolding, gene prediction, scripts |